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KMID : 0616620000060020391
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Journal of Soonchunhyang Medical College
2000 Volume.6 No. 2 p.391 ~ p.400
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The Inhibitory Effect of Ramulus Mori Extracts on Tyrosinase Activity in Normal Human Melanocytes and B-16 Melanoma Cells
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Abstract
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Background : Recently, the undesirable effects of UV exposure have been increasing because of destruction of ozone layer and excessive solar exposure in enjoying leisure. Therefore, the chance to have uneven skin pigmentations has been increasing.
To keep away from the unwanted skin pigmentation, melanogenesis inhibitors have been developed for use in cosmetic preparations for the purpose of skin whitening. Plant extracts having an inhibitory effect on melanin synthesis may be a good choice as a cosmetic ingredient because they have relatively fewer side effects.
Objective : In this study, the inhibitory effects of ramulus mori (young twigs of Morus alba L) on tyrosinase activity were investigated cultured normal human melanocytes and B-16 melanoma cells by using enzyme assay and RT-PCR.
Methods : Tyrosinase activity was determined by spectrophotometry. The effects of whitening agents (kojic acid, arbutin, licorice extracts and ramulus mori extracts) on mushroom tyrosinase was compared by measuring the IC50, the concentration of the compound at which half of the original tyrosinase activity is inhibited.
Normal human melanocytes taken from neonatal foreskin and B-16 melanoma cells, tyrosinase activity inhibition was measured by spectrophotometry. We observed tyrosinase volume in B-16 melanoma cells by using RT-PCR.
Results : Ramulus mori extracts showed strong inhibitory effect on tyrosinase activity in both normal human melanocytes and B-16 melanoma cells.
We also confirm that they have a inhibitory effect on tyrosinase expression in the RT-PCR
Conclusion : This study showed that ramulus mori extracts had strong inhibitory effect against tyrosinase activity. The results suggests that ramulus mori extracts can be used as a new whitening agent.
Key words: Ramulus mori, Tyrosinase Activity, RT-PCR
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